1 00:00:09,009 --> 00:00:07,119 plants growing in space have to cope 2 00:00:11,020 --> 00:00:09,019 with the physical realities of a 3 00:00:13,299 --> 00:00:11,030 microgravity environment where the lack 4 00:00:15,789 --> 00:00:13,309 of buoyancy driven convection can cause 5 00:00:17,890 --> 00:00:15,799 low oxygen conditions around the plant I 6 00:00:19,870 --> 00:00:17,900 spoke with one researcher who has grown 7 00:00:23,650 --> 00:00:19,880 plants on the space station inside a 8 00:00:25,090 --> 00:00:23,660 unique container what is brick it's not 9 00:00:27,880 --> 00:00:25,100 a brick like we think about on our house 10 00:00:31,030 --> 00:00:27,890 right though it's not brick means 11 00:00:33,760 --> 00:00:31,040 biological research in canister and it's 12 00:00:35,560 --> 00:00:33,770 a type of NASA hardware that's often 13 00:00:38,950 --> 00:00:35,570 been used to fly plants to the 14 00:00:41,650 --> 00:00:38,960 International Space Station there's five 15 00:00:45,160 --> 00:00:41,660 petri plates inside the shoe box sighs 16 00:00:47,740 --> 00:00:45,170 brick those petri plates are held in a 17 00:00:50,020 --> 00:00:47,750 petri dish fixation unit and the five 18 00:00:51,880 --> 00:00:50,030 petri plates also have a data logger 19 00:00:54,130 --> 00:00:51,890 which records the temperature inside the 20 00:00:56,110 --> 00:00:54,140 brick when you talk about growing plants 21 00:00:58,300 --> 00:00:56,120 in space this really excites people how 22 00:01:00,580 --> 00:00:58,310 did you get involved the growing lab is 23 00:01:02,970 --> 00:01:00,590 interested in studying plants responses 24 00:01:06,399 --> 00:01:02,980 to the environment and part of our 25 00:01:09,100 --> 00:01:06,409 expertise is looking at calcium changes 26 00:01:12,160 --> 00:01:09,110 in plant cells as they respond to 27 00:01:15,610 --> 00:01:12,170 different stresses so for example during 28 00:01:17,740 --> 00:01:15,620 low oxygen stress which is common on 29 00:01:20,050 --> 00:01:17,750 earth during flooding when flooding 30 00:01:22,750 --> 00:01:20,060 displaces the air spaces from the soil 31 00:01:26,110 --> 00:01:22,760 the roots experience low oxygen stress 32 00:01:28,510 --> 00:01:26,120 and this causes a lot of changes in gene 33 00:01:33,490 --> 00:01:28,520 expression and the ability for the plant 34 00:01:37,359 --> 00:01:33,500 to grow well so that naturally grew into 35 00:01:40,359 --> 00:01:37,369 the idea well if we can study plants on 36 00:01:42,969 --> 00:01:40,369 the space station in microgravity then 37 00:01:44,859 --> 00:01:42,979 there'll be some parallels to how plants 38 00:01:47,649 --> 00:01:44,869 are experiencing low oxygen stress on 39 00:01:49,210 --> 00:01:47,659 earth overall we've done a lot of these 40 00:01:50,890 --> 00:01:49,220 what have we learned what's the most 41 00:01:53,440 --> 00:01:50,900 important thing that we can take away so 42 00:01:55,570 --> 00:01:53,450 far after the plants are returned to 43 00:01:57,850 --> 00:01:55,580 earth we can study differences in gene 44 00:02:00,130 --> 00:01:57,860 expression so in addition to growing the 45 00:02:02,499 --> 00:02:00,140 plants on the space station we also grew 46 00:02:05,880 --> 00:02:02,509 plants at Kennedy Space Center on the 47 00:02:08,589 --> 00:02:05,890 earth to as a control so we can compare 48 00:02:10,839 --> 00:02:08,599 in parallel what's going on in the 49 00:02:13,629 --> 00:02:10,849 different environments and it turns out 50 00:02:14,110 --> 00:02:13,639 in space not only are the low oxygen 51 00:02:16,000 --> 00:02:14,120 stress 52 00:02:18,910 --> 00:02:16,010 genes upregulated like we had predicted 53 00:02:21,820 --> 00:02:18,920 but also a number of defense related 54 00:02:24,940 --> 00:02:21,830 genes including plant hormone signaling 55 00:02:28,390 --> 00:02:24,950 such as jasmonic acid and salicylic acid 56 00:02:31,270 --> 00:02:28,400 signaling genes are upregulated along 57 00:02:34,210 --> 00:02:31,280 with reactive oxygen species so all of 58 00:02:36,130 --> 00:02:34,220 these categories of genes indicate the 59 00:02:37,569 --> 00:02:36,140 plants are experiencing stress when 60 00:02:40,809 --> 00:02:37,579 they're in space flight but they're 61 00:02:42,640 --> 00:02:40,819 using the tools they have at hand the 62 00:02:44,530 --> 00:02:42,650 changes in gene expression to be able to